Antibacterial Activity Test Gynura procumbens (Lour.) Merr.( of Sambung Nyawa) Leaf Extract Against Escherichia coli

english

Abstract

Introduction: Diarrhea is a disease that contributes to the highest death rate in Indonesia. The number of diarrhea cases recorded in Indonesia in 2021 was 7.3 million. The most common cause of diarrhea is Escherichia coli bacteria. Based on previous studies, one of the traditional medicines that can treat diarrhea is sambung nyawa leaf (Gynura procumbens (Lour.) Merr.), which has various secondary metabolite compounds that can kill bacteria.

Objectives and Methods: This study aimed to determine the antibacterial activity of sambung nyawa leaf against Escherichia coli. The method used for extracting sambung nyawa leaf was the ultrasonic method. Antibacterial activity testing uses the well-diffusion method and the determination of the Minimum Inhibitory Concentration (MIC) and the determination of Minimum Bactericidal Concentration (MBC) by the dilution method. Test results data were analyzed using the Kruskal-Wallis Test.  

Results: of this research showed that sambung nyawa leaf has antibacterial activity through well-diffusion testing with an average inhibition zone at a concentration of 100% of 18.215 mm, a concentration of 75% of 16.02 mm, a concentration of 50% of 14.445 mm, a concentration of 25% of 13.33 mmm. The MIC test obtained the minimum inhibition ability at a concentration of 50%. Results: of the Kruskall-Wallis test in the three tests showed significant differences in each treatment group.

Conclusions: This research concludes that sambung nyawa leaf has antibacterial activity against Escherichia coli with a MIC value at a concentration of 50%. However, no MBC has been found against Escherichia coli.