Antibacterial Activity Test of Sambung Nyawa Leaf Extract (Gynura procumbens L.) Against Klebsiella pneumonia bacteria

english

Abstract

Introduction: Infectious diseases are one of the health problems that have developed over time. Infections are generally caused by various microorganisms, such as bacteria, viruses, fungi, and protozoa. One of the bacteria that causes infection is Klebsiella pneumonia. Gram-negative bacteria can cause urinary tract infections and respiratory infections, especially in individuals with weak immune systems. Based on previous research, one traditional medicine used to relieve pneumonia symptoms is the sambung nyawa leaf (Gynura procumbens (Lour) Merr.), which has various secondary metabolite compounds that can kill bacteria.

Objectives and Methods: This study aimed to determine the antibacterial activity of sambung nyawa leaf against Klebsiella pneumonia. The method used for extracting sambung nyawa leaf was the ultrasonic method. Antibacterial activity testing uses the well-diffusion method and the determination of the Minimum Inhibitory Concentration (MIC) and the determination of Minimum Bactericidal Concentration (MBC) by the dilution method. Test results data were analyzed using the Kruskal-Wallis Test.

Results: of this research showed that sambung nyawa leaf has antibacterial activity through well-diffusion testing with an average inhibition zone at a concentration of 60% of 12,9 mm, a concentration of 50% of 11,7 mm, a concentration of 40% of 9,8 mm, a concentration of 30% of 9,0 mmm. The MIC test obtained the minimum inhibition ability at a concentration of 40%. Results: of the Kruskall-Wallis test in the three tests showed significant differences in each treatment group.

Conclusions: This research concludes that sambung nyawa leaf has antibacterial activity against Escherichia coli with a MIC value at a concentration of 40%. However, no MBC has been found against Klebsiella pneumonia